Journal: bioRxiv

Article Title: δ-catenin controls layer-specific transcriptional maturation of astrocytes via Zbtb20

doi: 10.64898/2026.05.12.724361

Figure Lengend Snippet: A) PCA plots showing segregation of visual cortex samples by sex (A) and genotype (A’). B) PCA plots showing segregation of prefrontal cortex samples by sex (B) and genotype (B’). C) Volcano plot of DEGs in the prefrontal cortex of KO (N=6, 3 males, 3 females) compared to WT (N=6, 3 males, 3 females). Criteria for DEG selection: log2 fold change (FC) less than -0.15 or greater than 0.15; false discovery rate (FDR) < 0.05 as calculated by DESeq2 with Benjamini–Hochberg’s correction. D) RNA sequencing reads aligned to Ctnnd2 genomic DNA sequence. Reads aligned to exons 4 and 5 are absent in KO samples (red rectangle). E) Protein translation of KO allele showing early stop codon with excision of C tnnd2 exons 4 and 5.

Article Snippet: Target chromatin was isolated using the CUTANATM ChIC/CUT&RUN Kit Version 5 (EpiCypher 14-1048) or CUTANATM meCUT&RUN Kit for DNA Methylation Sequencing (Epicypher 14-1060-24), according to manufacturer instructions.

Techniques: Selection, RNA Sequencing, Sequencing